Oncology Letter, 2015, 01, 2603─2609

MicroRNA-650 in a copy number-variable region regulates the production of interleukin 6 in human osteosarcoma cells

JunHo Yun, Sanghoon Moon, Heun-Sik Lee, Mi Yeong Hwang, Yeon-Jung Kim, Ho-Yeong Yu, Youngsoo Kim, Bok-Ghee Han, Bong-Jo Kim, Jeong-Min Kim

Abstract

Copy number variation is a well？known genetic variation. microRNAs (miRNAs/miRs) are non？coding RNAs that mediate gene expression by regulating target mRNAs. In the present study, copy number deletions encompassing miRNA coding regions were investigated to determine the association between the deletion of miRNA and its phenotypic effects. A total of 38 human miRNAs in copy number variants were identified and miR？650, which is functional in the human osteosarcoma MG？63 cell line, was selected.
Overexpression of miR？650 decreased the expression of inhibitor of growth family member 4 (ING4) in the MG？63 cells and increased interleukin (IL)6 tranion, as well as IL6 secretion in IL1B？stimulated cells. Furthermore, miR？650 downregulated the amount of nuclear factor of κ light polypeptide gene enhancer in B cells inhibitor α and increased the tranional activity of nuclear factor (NF)κB. Downregulation of ING4 also increased the production of IL6, similar to miR？650 overexpression. Taken together, these data indicate that miR？650 plays a significant role in the production of IL6 by regulating ING4 expression and NFκB signaling in IL1B？stimulated MG？63 osteosarcoma cells

• ISBN or ISSN: 1792-1074

• 본 연구는 질병관리본부 연구개발과제(과제번호 2013-NG73001-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2013-NG73001-00) by Research of Korea Centers for Disease Control and Prevention.