Biomolecules and therapeutics, 2014, 01, 41─46

Antiviral Activity of Hederasaponin B from Hedera helix against Enterovirus 71 Subgenotypes C3 and C4a

Sang-Gu Yeo, Jaehyoung Song, E Hong,Y Kwon,H Kim,S Lee,J Park,H Ko

Abstract

Enterovirus 71 (EV71) is the predominant cause of hand, foot and mouth disease (HFMD). The antiviral activity of hederasaponin B from Hedera helix against EV71 subgenotypes C3 and C4a was evaluated in vero cells. In the current study, the antiviral activity of hederasaponin B against EV71 C3 and C4a was determined by cytopathic effect (CPE) reduction method and western blot assay. Our results demonstrated that hederasaponin B and 30% ethanol extract of Hedera helix containing hederasaponin B showed significant antiviral activity against EV71 subgenotypes C3 and C4a by reducing the formation of a visible CPE. Hederasaponin B also inhibited the viral VP2 protein expression, suggesting the inhibition of viral capsid protein synthesis.These results suggest that hederasaponin B and Hedera helix extract containing hederasaponin B can be novel drug candidates with broad-spectrum antiviral activity against various subgenotypes of EV71

• ISBN or ISSN: 1976-9148

• 본 연구는 질병관리본부 연구개발과제(과제번호 2013-NG48001-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2013-NG48001-00) by Research of Korea Centers for Disease Control and Prevention.