2013 기초의학 학술대회, 2013, 02, 238─238

Comparison of Whole Genome Sequence of Novel Human Respiratory Syncytial Virus (HRSV) ON1 Strain with Prototype A2 strain

You-Jin Kim, Wan-Ji Lee, Han Saem Lee, Ho Yeon Lee, Dae-won Kim, Kisoon Kim

Abstract

Human respiratory syncytial virus (HRSV) has been recognized as the most important viral agent of severe respiratory tract diseases in the pediatric and elderly people. This virus contains 15.2 kb length negative single strand RNA genome encoding 11 proteins. Among them, a viral attachment glycoprotein (G) forms the basis for subgroup classification of HRSV into A and B as the G proteins show genetic variations and different antibody cross reactivity. Continuously there has been a report about new genotypes in A and B subtype, 10 HRSV A genotypes, GA1 to GA7, SAA1, NA1 , NA2, and 13 HRSV B genotypes, GB1 to GB4, SAB1 to SAB3, BA1 to BA6, were identified. In addition, novel genotype ON1 belong to A subtype having 72 nucleotide duplication in G gene was discovered in Canada, 2010-2011 winter season. This duplication in the C-terminal hyper-variable region induced 24 amino acid lengthened G protein and may affect the antibody recognition. In this study, we also identified this new ON1 type in South Korea, 2011-2012 season and investigated the complete genome sequence of 15,277 bp (accession no. JX627336). From this whole genome sequences, genetic characteristics of 11 proteins and non-coding region were compared with previously reported HRSV genome sequences including A2 strain. M2-2 and G protein show low amino acid identity of 84.1% and 89.1 % whereas M and M2-1 protein of 100 % and 99 % high identity when compared to A2 strain. Epitope predictions of G proteins were also conducted and in that analysis several common epitopes and some unique epitopes also suggested. These data provide an update on sequences information of HRSV which little whole genome sequences are available and fundamental to study antigenic variability. However, further study to understand the association of virulence change, repeated infections and epidemiology with G gene duplication is needed.

• 본 연구는 질병관리본부 연구개발과제(과제번호 2013-NG47001-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2013-NG47001-00) by Research of Korea Centers for Disease Control and Prevention.