2013 추계 대한면역학회, 2013, 02, P-150─P-150

Characterization of SodB, a Cytoplasmic Superoxide Dismutase from Burkholdeira pseudomallei.

Yong-Woo Shin, Pil-Gu Park, Sang-Hwan Seo, Kee-Jong Hong, Gi-eun Rhie, Min-Hee Cho

Abstract

B.pseudomallei is a facultative intracellular pathogen. The ability for survival and proliferation of bacteria themselves inside eukaryotic cells is considered mostly important for the pathogenesis of melioidosis, also for the recrudescent nature of the disease and difficulties in treatment. Mostly bacteria may possess evasive mechanisms with antioxidant enzymes against oxidative killing fields raised by macrophages. However, there is no available information regarding the antioxidant enzymes of B.pseudomallei for the immune escaping. Superoxide, one of these oxidative environments, is an important mediator of pathogen scavenging. We identified the B.pseudomallei sodB gene which codes the protein for superoxide dismutase activity, and constructed an allelic deletion mutant of this gene from wild type bacteria. This mutant strain with reduced expression of sodB gene exhibited decreasing activity of SODB and increasing sensitivity to paraquat compared to wild-type bacteria. Furthermore, the mutant strain was more sensitive to killing process by extracellular superoxide than wild type strain, but not to superoxide generated as intracellular a component. The sodB mutant showed a markedly decreased survival cultured in RAW 264.7 murine macrophages. Through this study, cloning and expression of SODB from B. pseudomallei may provide information for further study about the biochemical characteristics of the enzyme, and its possible functional role in the pathogenesis of B.pseudomallei.

• 본 연구는 질병관리본부 연구개발과제(과제번호 2013-NG45002-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2013-NG45002-00) by Research of Korea Centers for Disease Control and Prevention.