2013 한국미생물학회연합 국제학술대회, 2013, 02, 319─319

Correlation of Three-point Titration Assays for Human Respiratory Syncytial Virus

김승태, 정향민, 김유진, 강혜지, 최기주, 남정구, 김기순

Abstract

Standardization of viral pathogen resource contributes to make them useful for research fields in medical science and laboratory healthcare. To grant a value with viral pathogen resource, standardization procedures are required to evaluate virus titer which represents infectivity and stability. Quantification of the number of infectious viral particles (plaque assay and TCID50 endpoint dilution assay) or the genetic material (qRT-PCR assay) associated with the virus is frequently utilized to decide virus titration. Each of above assay system has recruited unique biological characteristic of target viruses, this often leads numerical discordances of virus titers when different assays were used. In this study, three-point comparison of virus titers resulted from plaque assay, TCID50 endpoint assay and qRT-PCR assay was investigated using respiratory syncytial virus genotype A. There was more or less difference of virus titer when TCID50 was compared with PFU. In the other hands, significant difference was observed when virus titers PFU and TCID50 were compared with viral genome copy numbers. Although there were numerical differences in inter-assays, consistency of relative virus titers between assays were established. These data will be beneficial to the standardization of viral pathogen resource as well as characterization of biological properties of the virus.

• 본 연구는 질병관리본부 연구개발과제(과제번호 2013-NG47002-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2013-NG47002-00) by Research of Korea Centers for Disease Control and Prevention.