2013년 기초의학 학술대회, 2013, 02, 224─224

Development of Reference Strains for Bactericidal Efficacy Tests of Commercial Disinfectants/Sanitizers

A-Reum Yu, Na-Ri Shin, Kwang-Jun Lee

Abstract

Strains originated from American type Culture Collection (ATCC) are currently used as reference strains in general test methos. Korean food Additives Codex presents standardization test methods for assessing the effect of commercial sanitizers/disinfectant in Korean which is composed of 3 test methods such an in vitro suspension test, a surface test, and spore suspension test. The methods adapt Escherichia coli ATCC11229, staphylococcus aureus ATCC6538 and Bacillus subtilis ATCC6633 as reference strains. Therefore, the aim of this study is to develop reference strains with Korean cliical isolates for replacement of ATCC strains. From more than 500 strains registered in National Culture Collection for Pathogens (NCCP), 18 candidate strains were selected by bactericidal effect, biochemical test and 16s tRNA sequence analysis. Their sensitivity was tested to six representative sanitizer/disinfectants including ethanol, n-Alkyl(C12-C18)benzyldimethly Chloride, sodium hypochlorite, peroxyacetic acid, iodine and citric acid and the the results were compared with ones of the ATCC references strains. The strains showing same sensitivities with a reference at 100%, 50% and 25% of their maximum permissible concentrations based on Korean food additives codex was finally selected as the alternative to ATCC reference strain, which was confirmed by observing their similar morphologic changes on exposure to sanitizers/disinfectants under electronic microscope. thus, we suggest than E.coli NCCP14134, S. aureus NCCP14339 and B.subtilis NCCp10827 could be applied as the reference strains for authorized test methods. These trials also have significant meaning in therms of development and practical use of domestic bioresources

• 본 연구는 질병관리본부 연구개발과제(과제번호 2013-NG45006-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2013-NG45006-00) by Research of Korea Centers for Disease Control and Prevention.