2013년도 생화학분자생물학회 연례국제학술대회, 2013, 02, 114─114

Berberine inhibits adipogenesis via ATF3- mediated Pref1 regulation

JiYeon Kim, Keon Jae Park, Dae Yeon Lee, Gyu Hee Kim, Eun Ae Jeong, Won-Ho Kim

Abstract

Obesity is one of the most epidemic diseases. Usually, obesity is caused by energy unbalance: high calorie intake but low energy expenditure and closely associated with severe pathologies such as type 2 diabetes, hyperlipidemia, hypercholesterolemia and hypertension.
Berberine(BBR), a quaternary ammonium salt, is a natural compound which has been used in various purposes. In spite of many experiments, however, the mechanism by which BBR relieves obesity still remains unknown.
Here, we present the evidences that BBR directly impacts on adipogenesis. First, BBR inhibits lipogenesis via regulation of lipogenic genes expression. Second, this regulation is dependent on expression of ATF3 which is a stress inducible tranion factor. Third, ectopic expression of ATF3 during adipogenesis gives rise to the increased expression of Pref-1 and its downstream protein Sox9 that is usually appeared in preadipocyte. Finally, interrupted adipogenesis by BBR is partially recovered by reduction of ATF3 or Pref-1 expression.
From these data we propose new mechanism by which BBR relieves obesity through inhibition of adipogenesis, especially via tranional regulation. This finding will facilitate the study to develop the new medicine using BBR

• ISBN or ISSN: 0000-0000

• 본 연구는 질병관리본부 연구개발과제(과제번호 2011-NG64001-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2011-NG64001-00) by Research of Korea Centers for Disease Control and Prevention.