2013 International conference of the korean society for molecular and cellular biology, 2013, 02, 119─119

Toll-like receptor 2 and 4 contribute to Yersinia pestis-mediated inflammatory response in mouse dendritic cells

JunHo Jeon, Ok Kyu Park, Sung Yeon Kim, and Gi-eun Rhie

Abstract

Y. pestis, the etiological agent of plague, is a Gram-negative, rod-shaped, facultative anaerobic bacterium that causes a life-threatening infectious disease to humans and other animals. Here, we investigated the effect of Y. pestis infection on dendritic cells (DCs) which play a pivotal role in appropriate activation of host immune system. When mouse bone marrow-derived DCs were stimulated with Y. pestis grown at 27˚C or 37C, the DCs became mature with an increase in the expression of maturation markers such as CD80, CD86, and MHC class II and did not show any differences in that of maturation markers. Concomitantly, infection of DCs with Y. pestis significantly induced production of proinflammatory cytokines and chemokines such as TNF-α, IL-6, MCP-1, and IP-10 in a bacterial concentration-dependent manner. However, TLR2-/- or TLR4-/- DCs infected with Y. pestis showed reduced production of proinflammatory cytokines including TNF-α, and IL-6 compared with those of wild-type mice. Additionally, purified fraction 1 (F1) capsule protein and lipopolysaccharide (LPS) from Y. pestis were able to stimulate TLR2 and TLR4 activation, respectively. These results suggest that TLR2 and TLR4 could contribute to Y. pestis-induced inflammatory responses in DCs.

• ISBN or ISSN: 0000-0000

• 본 연구는 질병관리본부 연구개발과제(과제번호 2012-N45002-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2012-N45002-00) by Research of Korea Centers for Disease Control and Prevention.