Osong Public Health Res Perspect, 2014, 02, 345─350

Aging-related Changes in Mouse Serum Glycerophospholipid Profiles

Seung woo Kim, Hyo-Soon Cheon, Jae-Chun Song, Sang-Moon Yun, Sang Ick Park, Jae-Pil Jeon

Abstract

Objectives
Metabolic dysfunction is a common hallmark of the aging process and aging-related pathogenesis. Blood metabolites have been used as biomarkers for many diseases, including cancers, complex chronic diseases, and neurodegenerative diseases.
Methods
In order to identify aging-related biomarkers from blood metabolites, we investigated the specific metabolite profiles of mouse sera from 4-month-old and 21-month-old mice by using a combined flow injection analysisetandem mass spectrometry and liquid chromatographyetandem mass spectrometry.
Results
Among the 156 metabolites detected, serum levels of nine individual metabolites were found to vary with aging. Specifically, lysophosphatidylcholine (LPC) acyl (a) C24:0 levels in aged mice were decreased compared to that in young mice, whereas phosphatidylcholine (PC) acyl-alkyl (ae) C38:4, PC ae C40:4, and PC ae C42:1 levels were increased. Three classes of metabolites (amino acids, LPCs, and PCs) differed in intraclass correlation patterns of the individual metabolites between sera from young and aged mice. Additionally, the ratio of LPC a C24:0 to PC ae C38:4 was decreased in the aged mice, whereas the ratio of PC ae C40:4 to LPC a C24:0 was increased, supporting the aging-related metabolic changes of glycerophospholipids.
Conclusion
The ratios of the individual metabolites PC and LPC could serve as potential biomarkers for aging and aging-related diseases.

• ISBN or ISSN: 2210-9099

• 본 연구는 질병관리본부 연구개발과제(과제번호 2012-NG62002-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2012-NG62002-00) by Research of Korea Centers for Disease Control and Prevention.