Biochemical and Biophysical Research Communications, 2014, 01, 549─555

Identification of anti-allergic effect of Clonorchis sinensis-derived protein venom allergen-like proteins (CsVAL)

Young-Il Jeong, Yu-Jung Kim, Jung-Won Ju, Sung-Hee Hong, Myoung-Ro Lee, Shin-Hyeong Cho, Won-Ja Lee, Sang-Eun Lee

Abstract

allergic responses. We investigated the effects of C. sinensis venom allergen-like (CsVAL) proteins on immune-modulating activities in allergic inflammatory response. Using RBL-2H3 rat mast cells, we demonstrated that CsVAL inhibits antigen-induced b-hexosaminidase release from immunoglobulin E-sensitized RBL-2H3 cells, and this inhibitory activity occurs by suppressing Lyn phosphorylation and intracellular reactive oxygen species production. In addition, CsVAL peptide treatment inhibits activation of protein kinase C-a and extracellular signal-regulated kinase 1/2, which are involved in degranulation of immunoglobulin E-sensitized mast cells. Furthermore, immunization with CsVAL suppressed development of skin inflammation by assessing ear thickness and cutaneous infiltration by eosinophils and mast cells in oxazolone-induced contact hypersensitivity in vivo mouse model. These results suggest that CsVAL is a promising candidate as an effective mast cell inhibitor for allergic and inflammatory diseases.

• ISBN or ISSN: 0006-291X

• 본 연구는 질병관리본부 연구개발과제(과제번호 2011-N54001-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2011-N54001-00) by Research of Korea Centers for Disease Control and Prevention.