2013년도 생화학분자생물학회 연례국제학술대회, 2013, 02, 114─114

Metabolome analysis of the STZ-injected rat urine by CE-TOFMS

DaeYeon Lee, Gyu Hee Kim, Keon Jae Park, Eun Ae Jeong, Ji Yeon Kim, WonHo Kim

Abstract

In our studies, the kidney of streptozotocin-injected rat (type 1 diabetes model) showed the typical pathophysiological features of diabetic nephropathy such as mesangial matrix expansion and renal fibrosis. To identify biological markers of DN and for further functional studies about remarkable metabolite, we performed metabolome analysis of the rat urine by capillary electrophoresis time-of-flight mass spectrometry (CE-TOFMS), which is an extremely high resolution method. Urine samples from 3 controls and 3 STZ-injected rats were analyzed in both cation and anion mode. Principal component analysis showed that two groups are clearly clustered. 248 putative metabolites were detected and the quantities of 108 metabolites among them were estimated. Total 28 metabolites showed significant differences (p-value <0.05) in their quantities in both groups- 21 metabolites were increase(>3-fold; 4, >2-fold; 7) and 7 metabolites were decreased in STZ-rat urine. We are going to test the potential of these metabolites as biomarkers of DN. In addition we plotted the putative metabolites on the pathway map including glycolysis, pentose phosphate, TCA cycle, urea cycle, purine, pyrimidine, nicotinic acid, nicotinamide and amino acid pathways in order to further functional studies.

• 본 연구는 질병관리본부 연구개발과제(과제번호 2013-NG64002-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2013-NG64002-00) by Research of Korea Centers for Disease Control and Prevention.