2013 한국분자ㆍ세포생물학회 동계학술대회, 2013, 02, 36─36

Activating Tranion Factor-3 plays as a novel potent regulator of Renal Fibrosis in Streptozotocin-injected rats

GyuHee Kim, JeongEun Kim, Do Hee Kim, JiYeon Kim, Jeong Suk Kang, Keon Jae Park, Eun Ae Jung, WonHo Kim

Abstract

Diabetic nephropathy, an end-stage disease of diabetic complication, is characterized by fibrosis of renal glomerulus and tubulointerstitial region. However, the exact molecular mechanisms by which diabetes may initiate or exacerbate kidney failure remain elusive. Here, we investigated the role of ATF3 on theinduction of diabetic nephropathy and molecular mechanisms involved in ATF3-mediated fibrosis. STZ-injected rats exhibited renal dysfunction, as evidenced by increased glomerulus, thickened basement membrane, and increased mesenterium, which are consistent with higher levels of albuminuria,blood glucose and serum MCP-1. The infiltration of CD68+ cells was also significantly increased in the glomerulus and tubulointerstitial region, correlated with the marked accumulation of ECM molecules and ATF3. In NRK-52E cells, TNF-？-induced fibrosiswas determined by ATF3 since the increasedECM molecules such as MCP1, FN, collagen IV are abolished by ATF3 depletion. Also, ATF3 plays as a direct tranional activator of MCP-1 gene. Collectively, we know that ATF3 may play as an important regulator of renal fibrosis and is considered as a new aspect of the therapeutic mechanism of DN.

• ISBN or ISSN: 0000-0000

• 본 연구는 질병관리본부 연구개발과제(과제번호 2013-NG64002-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2013-NG64002-00) by Research of Korea Centers for Disease Control and Prevention.