2013 한국응용곤충학회 추계학술대회, 2013, 02, 168─168

Wolbachia infection according to geographical location in Aedes albopictus (Diptera: Culicidae) in Korea

ChanHee Park, HyungWoo Lim, HyunWoo Kim, Mi Yeoun Park, Wook-Kyo Lee, Jong Yul Roh, Kyu Sik Chang, and E-Hyun Shin

Abstract

The intracellular endosymbiont bacterium Wolbachia is currently considered the most abundant in arthropods. Many Wolbachia manipulate host reproductive systems; it lead to male-killing; cytoplasmic incompatibility; parthenogenesis and feminization of genetic males with large impact on host ecology and evolution in arthropods. Recently; Wolbachia-based research was focused on the control of disease vector-population; such as several mosquito such as genus Aedes and Anopheles which cause dengue fever and malaria; respectively. In this study; we investigated the distribution of Wolbachia infection in Ae. albopictus according to geographical distribution. We selected 9 areas and collected Ae. albopictus at least 30 specimens in each area; these areas were distinguished with mountain chain and waterway. Whole genomic DNA were extracted from collected specimens and PCR analysis were accomplished in each specimen using Wolbachia specific primers such as 16S and WSP genes. As a results; two Wolbachia marker genes are showed same detection patterns and infection rates are 80~100% according to regional different. Our results indicated that Ae. albopictus are commonly infected with Wolbachia; it is possible that Wolbachia may act as endosymbiont in Ae. albopictus regardless of geographical region. In these results supported that dengue virus infection would rare with Ae. albopictus because of Wolbachia infected mosquitos are suitable for dengue virus. Although; low infection rate of Wolbachia; we need continuous survey between Wolbachia and Ae. albopictus as a vector of dengue fever.

• 본 연구는 질병관리본부 연구개발과제(과제번호 2013-NG55002-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2013-NG55002-00) by Research of Korea Centers for Disease Control and Prevention.