Journal of antimicrobial chemotherapy, 2013, 01, 2170─2172

Nosocomial transmission of NDM-1-producing Escherichia coli ST101 in a Korean hospital

JungSik Yoo, Hye Mee Kim, Hyun Sook Koo, Ji Woo Yang, Jae Il Yoo, Hwa Su Kim, Hye Kyung Park and Yeong Seon Lee

Abstract

Clonally diverse NDM-1-producing Escherichia coli (ST405, ST131, ST156 and ST101) have been reported globally, including on the Indian subcontinent.1 Indeed, there may be an association between isolates of NDM-1-producing E. coli ST101, phylogroup B1, that have been identified in Australia, Canada, Germany, the UK and Pakistan.2 In December 2011, NDM-1-producing E. coli (CREC-36) was identified from a patient who was hospitalized in a 260 bed tertiary care hospital in Korea. Medical records were reviewed and 114 stool or rectal swab samples were taken from 67 patients on Ward 1 (19 rooms), 11 patients on the intensive care unit (ICU) and 25 healthcare workers (doctors, nurses and caregivers) working on Ward 1. We screened patients in the ICU because the first patient had been treated in the ICU for 1 month before she was transferred to room 112. In addition, 23 environmental swabs were collected from washbasins, medicine trays and linen. All samples were cultured in 5 mL of tryptic soy broth containing 10 mg meropenem discs and processed using the CDC protocol.3 The modified Hodge test4 and the double-disc synergy test were performed on representative colonies grown on MacConkey agar. The blaNDM-1 gene was identified by PCR and sequencing using the primers NDM-1 F (5′-CAATATTATGC ACCCGGTCG-3′) and NDM-1 R (5′-ATCATGCTGGCCTTGGGGAA-3′)

• ISBN or ISSN: 0305-7453

• 본 연구는 질병관리본부 연구개발과제(과제번호 2011-N44001-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2011-N44001-00) by Research of Korea Centers for Disease Control and Prevention.