2013년 한국분자세포생물학회(KSMCB 2013), 2013, 02, 121─121

Effects of ATF3 on Berberine-inhibited adipogenesis and obesity

Ji Yeon Kim, Keon Jae Park, Dae Yeon Lee, Gyu Hee Kim, Eun Ae Jeong, Won-Ho Kim

Abstract

Obesity, as one of the most epidemic diseases, is known as a strong risk factor for T2DM. Berberine (BBR) is well known to have beneficial effects in the treatment of diabetes and obesity. Here, we have investigated the role of ATF3 and its downstream target regulator during BBR-inhibited adipocyte differentiation. BBR reduced body weight, plasma triglyceride, and plasma glucose or insulin levels and improved insulin action in high fat diet-fed rats. In BBR-treated adipose tissues, ATF3 was significantly increased along with the Pref-1 and its downstream target Sox9 that is highly expressed in preadipocyte. Concomitantly, BBR treatment resulted in decreased adipocyte differentiation and delayed the downregulation of pref-1 and sox-9 expression, which subsequently, leads to the reduction of adipogenic gene expression. ATF3-inhibited adipocyte differentiation was accomplished by increasing the expression of Pref-1 and Sox-9. We found that BBR-induced ATF3 may play as an upstream inhibitory regulator for the downregulation of Pref-1 and Sox-9, early markers of adipocyte differentiation, thus leads to the reduction of adipocyte differentiation and obesity.

• ISBN or ISSN: 0000-0000

• 본 연구는 질병관리본부 연구개발과제(과제번호 2011-NG64001-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2011-NG64001-00) by Research of Korea Centers for Disease Control and Prevention.