9th International Symposium on Antimicrobial Agents and Resistance (ISAAR), 2013, 02, 79─79

Binding capacity to epithelial cell and extracellular matrix components of vancomycin intermediate Staphylococcus aureus

JungWook Kim, Hyun Kyung Kim, Gi Su Kang, Hwa Su Kim, Jung Sik Yoo, Yeong Seon Lee, and Jae Il Yoo

Abstract

Background
Staphylococcus aureus is a major human pathogen responsible for superficial skin infections as well as for serious invasive infections. S. aureus has a variety of virulence factors. The important step in the initiation of invasive staphylococcal disease is adherence to host tissues and plasma proteins. In this study, to investigate the difference of adherence properties between VISA and vancomycin susceptible Staphylococcus aureus (VSSA), we measured the interaction of VISA and VSSA strains with epithelial cells and extracellular matrix (ECM) components.
Methods
The VISA and VSSA strains were characterized by MLST, agr typing. The adherence and invasion capacity of VISA and VSSA strains about the A549 human lung epithelial cells was investigated. To investigate the adherence of S. aureus to ECM components, human cellular fibronectin, human plasma fibronectin, type I collagen, type IV collagen, and laminin components were used. The statistical significance (P<0.05) compared with VSSA or VISA strains was calculated by Kruskal-Wallis test.
Results
Among VISA and VSSA strains, the VISA strains of ST5/agrII type had reduced binding capacity (20%) to epithelial cell compared to that of ST5/agrII VSSA (60%) strains. And there was no significant difference in invasion results. The binding capacity of VISA strains of ST5/agrII type was reduced than that of VSSA (ST5/agrII) strains to the human cellular fibronectin, type I collagen, type IV collagne, and laminin component. In addition to, this type of VISA strains also showed reduced binding capacity to type I collagen, type IV collagen and laminin component compared to ST1/agrIII, ST72/agrI, and ST239/agrI strains of VISA.
Conclusion
The ST5/agrII is the one of the representative VISA clone in Korea. This clone showed reduced binding capacity to epithelial cell and ECM component than that of VSSA and other VISA clone.

• ISBN or ISSN: 0000-0000

• 본 연구는 질병관리본부 연구개발과제(과제번호 2012-N44002-00) 연구비를 지원받아 수행되었습니다.
• This research was supported by a fund(code 2012-N44002-00) by Research of Korea Centers for Disease Control and Prevention.