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Frontiers in Genetics, 2019. 10, 884-, DOI: https://doi.org/10.3389/fgene.2019.00884
MUDENG Expression Profiling in Cohorts and Brain Tumor Biospecimens to Evaluate Its Role in Cancer
Shin J, Choi JH;Jung S;Jeong S;Oh J;Yoon DY;Rhee MH;Ahn J;Kim SH;Oh JW
Mu-2-related death-inducing gene (MUDENG, MuD) has been reported to be involved inthe tumor necrosis factor-related apoptosis-inducing ligand (TRAIL)-associated apoptoticpathway of glioblastoma multiforme (GBM) cells; however, its expression level, interactors,and role in tumors are yet to be discovered. To investigate whether MuD expressioncorrelates with cancer progression, we analyzed The Cancer Genome Atlas (TCGA)database using UALCAN and Gene Expression Profiling Interactive Analysis (GEPIA).Differential expression of MuD was detected in 6 and 10 cancer types, respectively.Validation performed using data from the Gene Expression Omnibus database showedthat MuD expression is downregulated in KIRC tumor and correlate with higher chanceof survival. Upregulation of MuD expression in GBM tumors was detected through GEPIAand high MuD expression correlated with higher survival in proneural GBM, whereas theopposite was observed in classical GBM subtype. GBM biospecimens analysis showsthat MuD protein level was upregulated in three of six specimens, whereas mRNA levelremained relatively unaltered. Therefore, MuD may exert differential effects accordingto subtypes, and/or be subjected to post-translational regulation in GBM. Correlationanalysis between GBM cohort database and experiments using GBM cell lines revealedits positive effect on regulation of protein phosphatase 2 regulatory subunit B’Epsilon(PPP2R5E) and son of sevenless homolog 2 (SOS2). STRING database analysis indicatedthat the components of adaptor protein complexes putatively interacted with MuD butshowed no correlation in terms of survival of patients with different GBM subtypes. Insummary, we analyzed the expression of MuD in publicly available cancer patient datasets, GBM cell lines, and biospecimens to demonstrate its potential role as a biomarkerfor cancer prognosis and identified its candidate interacting molecules.
- DOI: https://doi.org/10.3389/fgene.2019.00884
- ISBN or ISSN: 1664-8021
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- This research was supported by a fund by Research of Korea Centers for Disease Control and Prevention.